One of Camtech's early incubation partnerships, The Clarity™ digital PCR system aims to disrupt the high precision digital PCR market.
Digital PCR, also known as next-generation PCR, is a powerful new technique for detecting DNA with extremely high sensitivity and precision. It works by partitioning a typical PCR reaction into thousands of nanolitre sub-reactions such that each has at most a single copy of DNA. By counting the number of positive reactions after PCR, the number of copies of DNA present in the sample can be directly enumerated.
The unique chip-in-a-tube design combines the robustness of chip-based partitioning with the ease-of-use of a tube-strip. Each PCR mix is rapidly subdivided into 10,000 partitions by a high-density chip within each tube. The high-density chip allows a large number of partitions to exist within the small confines of a PCR tube. Partitioning can be completed within 1 minute and is done in-tube without multiple pipetting steps.
The nifty Clarity™ auto loader simplifies one of the most important steps in digital PCR – sample partitioning. Its clever design allows the user to easily load the PCR samples onto the disposable sample loading kit. Once all 8 samples are loaded, the single touch of a button automatically transfers the samples into the tube-strip and uniformly distributes each sample over the chip. The end result is that 8 samples are subdivided into approximately 10,000 partitions each. All these only in about a minute! Truly, sample loading and partitioning for digital PCR have never been easier.
Designed to work with the tube-strips, the Clarity™ reader allows fluorescent signals from every single partition of the digital PCR reactions to be detected with high sensitivity. Unlike droplet-based systems, partitions in each tube are detected concurrently, saving time and allowing tube-strips to be read in just minutes. Closed tube format prevents cross contamination among reactions. Walk away operation allows multiple reactions to be read automatically with no user intervention, providing an easy workflow for high throughput digital PCR experiments.
| ABSOLUTE QUANTIFICATION | Detects and measures nucleic acid copies with high precision and sensitivity |
| CLOSED TUBE FORMAT | Prevents cross contamination among reactions |
| CHIP-BASED PARTITIONING | Stable partitions minimises sample loss |
| EASY WORKFLOW | Unique chip-in-a-tube design simplifies digital PCR workflow |
| FAST | Performs up to 96 digital PCR reactions per run; Well-suited for high throughput analysis |
| FLEXIBLE | Compatible with conventional thermal cyclers; Optimised for both probe-based and EvaGreen® assays |
| AFFORDABLE | Competitively priced to fit most budgets |